ATG ACEMBL - AUTOMATABLE DNA

First assemble your gene clusters in E. coli then shuttle it to the expression system of your choice.

The DONOR-ACEPTOR based Gene Assembly System with High Efficiency and Variability for E. coli.

Here we describe in detail ACEMBL, a system for multi-gene expression in
E. coli that can be used with ease in a truly automated, standarized setup.
E. coli is still the dominant work-horse for recombinant expression, for many
good reasons ranging from low-cost to the availability of a multitude of
expression strains, each with its own merit.

ACEMBL applies tandem recombination steps for rapidly assembling many
genes into multigene expression cassettes. These can be poly-cistronic
or multiple expression modules, or a combination of these elements.

DNA sequences of ACEMBL vectors are provided in the Appendix and can be
copied from there for further use. Multi-protein complex expression is at the
fore-front of contemporary biology.

Several systems have been introduced to co-express proteins in a variety of hosts, each with its own merit. Current systems share in common that they rely, to a varying degree, on standard cloning procedures by restriction enzymes and ligases for the integration of expression elements and their arrangement into functional multi-gene expression vectors. These steps are labor intensive, cumbersome and require significant time and effort, as well as considerable molecular biology expertise for their application.
This is refractory to high-throughput, robotics applications and do not provide flexibility for rapid revision of expression experiments with altered components.
However, automation and flexibility, combined with a reasonable experimental timeframe are a top priority in contemporary protein science.