Multi-gene expression / multi-protein expression systems
Multi-protein assemblies dominate a host of biological processes such as transcription, translation, RNA splicing, signaling cascades, protein trasnport, trafficking and degradation, etc. Life science researchers have become increasingly interested in studying these complexes in more detail as they realize that no protein works in isolation in these processes and thus should ideally also not be studied in isolation when investigating its context-dependent function in cells.
Systems I: MultiLabel
Culturing cells that have been modified to express foreign genes has become one of the mainstays of functional investigations in cellular physiology and biochemistry and more so in the production of biologically relevant molecules, e.g. amino acids, hormones, etc.
Co-expression of proteins in mammalian cells has traditionally relied on co-transfection of vectors carrying one or maybe two gene expression cassettes. It becomes more challenging when you are trying to express three or more proteins. Yet, you often wish or need to simultaneously express a bunch of proteins, e.g. to analyze cellular localization, to study signal pathways in the cells or to reprogram e.g. stem cells to change their developmental fate as a prerequisite for future therapeutic applications in stem-cell therapy.
Culturing cells that have been modified to express foreign genes has become one of the mainstays of functional investigations in cellular physiology and biochemistry and more so in the production of biologically relevant molecules, e.g. amino acids, hormones, etc.
Co-expression of proteins in mammalian cells has traditionally relied on co-transfection of vectors carrying one or maybe two gene expression cassettes. It becomes more challenging when you are trying to express three or more proteins. Yet, you often wish or need to simultaneously express a bunch of proteins, e.g. to analyze cellular localization, to study signal pathways in the cells or to reprogram e.g. stem cells to change their developmental fate as a prerequisite for future therapeutic applications in stem-cell therapy.
Systems II: flexTEC systems
These are the ready-to-use versions of the Toggle concept for the construction and expression of multi-gene expression constructs.
These are the ready-to-use versions of the Toggle concept for the construction and expression of multi-gene expression constructs.