NExT - Protein Optimisation Platform

The NExT Gene Diversification Process in Progress:

• Genes are diversified by in vitro mimicking of molecular processes, which are used in Nature for the diversification of antibody populations.
• Like in Nature the resulting gene products (e.g. Enzymes) are analysed for favourable candidate molecules.
• ATG uses specially designed highly focussed BioAssays covering populations and high throughput screenings.
• References:
  • (1) Di Noia J, Neuberger MS (2002) Altering the pathway of immunoglobulin hypermutation by inhibiting uracil-DNA glycosylase. Nature 419(6902):43-8.
  • (2) Immunity through DNA deamination. Trends Biochem Sci. 2003 Jun;28(6):305-12.
  • (3) Nucleotide Exchange and Excision Technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution
  • (4) Software Download: Nucleotide Exchange and Excision Technology Simulation
    • NExTProg 1.0 for Windows: NExTprog.zip
    • NExTProg info file: NExTinfo.rtf
    • Sequence example file: cat1_wt.txt

Engineering Thermo - stability and Thermo - activity:[info]

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