production of your proteins - from µg to mg scale - based on experience.
For functional analyses, structural studies of targets or just for commercial use high yields of soluble protein are required. We developed a special medium and use an expression system, which was exactly designed for this purpose.
Busso et. al. (2005) Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherischia coli. Anal.Chem. 343:313-321
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