Molecular Biologist's Efficient Basic Tool Kits 

KISS - keep it simple and smart - Kits of the MOSAIC Series :

MOSAIC - series cloning kits are the most basic standard line for molecular engineering -
fast and reliable and high throughput simply designed to guarantee simple handling - nothing else.

1. FBR - Kit for easy and robust recombination of homologous DNA for functional improvements
2. ERM - homogeneous introduction of random point mutations
3. UFL - universal ligation - don't think about compatibility of ends
4. RFL - efficient and fast ligation - speed optimised
5. CFS - efficient and fast colony screening for recombinants and cloning efficiency
6. HCE or HEE - efficient transformation of E. coli cells
7. OSM - rich growth medium optimised for protein expression in E. coli - low foam, high solubility, less protein oxidation (designed on the basis of experience and expertise in culturing bacteria)

The MOSAIC-FBR-kit
FBR (Fragmentation Blending Reassembling) for functional improvements of gene products. Mixtures of homologous DNA populations are cut at random  (Fragmentation). DNA populations can be derived from selected gene families, by error prone PCR or by synthetic genes. Chemical cleavage at Thymidine  positions generates  highly complex heterogeneous blends of single stranded DNA sequences. (Blending). By reassembling DNA fragments recombination  events are induced. These are  suitable for new combinations of internal new point mutations to be formed (Reassembling). These events provide an increased  probability of generating functional complementations improving intended features of gene products like proteins or RNA. (Reorder # : KT060616FBR). In  combination with the ERM - Kit (Reorder  # : KT060616ERM) this powerful tool provides increased probability for functional improvements in gene products  like  protein or RNA. Lit.:Müller et.al. (2005)  Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation  and directed evolution. NAR 13:e117. Reorder #: KT060616FBR Reagents for 100 reactions - price: 249 Euro  Reagents for 50 reactions - price: 149 Euro  [Order]

The MOSAIC-ERM-kit
ERM : Error-prone Random Mutagenesis is designed for equally distributing point mutations into functional gene sequences. Transitions (Py→Py, Pu→Pu) and transversions (Py→Pu, Pu→Py) are intended to be induced at the same frequency. Starting from the DNA-level, random mutagenesis during molecular synthetic amplification induces increasing sequential errosions in integrity. Reorder # : KT060616ERM. In combination with the FBR - kit (Reorder # : KT060616FBR) powerful tools are provided for increasing the probability for functional improvements in gene products like protein or RNA. Cadwell and Joyce (1994) Mutagenic PCR. PCR Methods Appl. 6:136-40, Spee, J. et al. Nucleic Acids Research, 1993, Vol. 21, No. 3 777-778. Reorder #: KT060616ERM Reagents for 100 reactions Price: 150 Euro  [Order]

The MOSAIC-UFL-kit
UFL-standard kit (Universal Fragment Linking) for simple and fast ligation of any blend and all kinds of DNA fragments and cycling products of Polymerases (like Taq) and proof-reading polymerases (like Pfu or Pwo). This unifying kit is sufficient for making compatible blunt, sticky or 3'-T overhangs for ligation. Even large PCR products can be cloned. A cloning vector pFUC1 (2695 bp, pUC derivative, blue/white screening, M13 forward and reverse primer) is supplied linearized and activated for ligation for direct and efficient cloning in a minimum of time. Quality checked and certified. Reorder #: KT060616UFL Reagents for 10 reactions - price 140 Euro  Reagents for 20 reactions - price 238 Euro  [Order]

The MOSAIC-RFL-kit
RFL standard kit (Rapid Fragment Linking) for simple and fast ligation of any blend and all kinds of DNA fragments and cycling products of Polymerases (like Taq) and proof-reading polymerases (like Pfu or Pwo). This unifying kit is sufficient for making compatible blunt, sticky or 3'-T overhangs for ligation. Even large PCR products can be cloned. A cloning vector pFUC1 (2695 bp, pUC derivative, blue/white screening, M13 forward and reverse primer) is supplied linearized and activated for ligation for direct and efficient cloning in a minimum of time in most cases 1 min. Quality checked and certified. Most text, 'cause this is the really incredible kit whose name is RFL-kit and it is so beautiful and so on. Reorder #:KT060616RFL Reagents for 30 reactions Price without competent cells 79 Euro  Price with competent cells 285 Euro  [Order]

The MOSAIC-CFS-kit
CFS colony fast screening kit for recombinant clone-based plasmid size simple and fast detection of high- and low-copy E. coli plasmids and identification of recombinant plasmid inserts as well as fast calculation of cloning efficiencies. Minipreps are OUT. Screening is performed directly using the primary colonies after transformation. One colony is sufficient for screening. Results are achieved within less than one hour by simple agarose gel electrophoresis. Reorder #:KT060616CFS Reagents for 100 reactions with Taq master mix Price: 79 Euro   [Order]

The MOSAIC-HCE-kit
HCE highly chemo-competent E. coli cell: an everyday efficiency tool: chemically competent cells Remove your bottleneck. Cloning and transformation results are as good as the competent cells used. We provide you the reliable standard of high quality chemically competent cells. Reorder # : KT060616HCE Reagents for 30 transformations, not aliquoted - price 219 Euro  Reagents for 10 transformations, aliquots in single tubes - price 159 Euro  [Order]

The Medium-OSM-kit
Medium - OSM (Optimum Solubility Medium) - Rich medium to increase solubility of proteins and reduce foam during cultivation and recombinant expression in E. coli. During recombinant expression in the common host E. coli, many proteins have the tendency to aggregate as insoluble inclusion bodies. Since these proteins are not in the native and mostly desired conformation, difficult denaturation and renaturation steps are necessary to obtain a soluble protein with correct conformation. Unfortunately, these renaturation steps are very time consuming and results as well as the final yield could not be predicted. Therefore, the expression of a soluble and correctly folded recombinant protein is most likely desired. The medium "OSM" increases the amount of soluble protein during recombinant expression. OSM is available as ready-to-use liquid medium as well as powder for preparation of medium. Reorder #: KT060616OSM Powder for 1 litre - price 33 Euro  Powder for 5 litre - price 109 Euro  [Order]

Choose one of the following ways to order:

1. Use the ordering form below.

2. Call us (+49-761-8889424).

3. Email us @ info@atg-biosynthetics.de

4. Ask your purchasing department to fax us (+49-761-8889425) the valid purchase order number.

5. ATG:biosynthetics adopt a non-disclosure policy to all of our customers. To protect your proprietary information please mail, email (info@atg-biosynthetics.de) or fax (832-886-5152) your confidentiality agreement to us for signing. If intended, this should be done before any information is released to us. If you don't have your own NDA/confidentiality agreement,  you can find ours here. Feel free to modify it to suit your specific needs.

ATG:biosynthetic - Synthetic Biology Tools - Improved Functionality for Your Synthetic Biology Applications

[home]