Synthetic Genes & de novo DNA-Synthesis

Introduction:

Gene synthesis provides the freedom of creating any gene in the absence of genomic or cDNA. Extending the capability of oligonucleotide synthesis technology, gene synthesis is indispensable for new gene creation and artificial genome assembly. Gene synthesis involves multiple steps which, so far, precluded it from full automation. ATG:biosynthetics' Custom Gene Synthesis Service implements rigorous standards and incorporates many innovations at each of the many steps: from computer aided gene design, high fidelity amplification, restriction site independent and size unlimited gene assembly to efficient subcloning, which makes our service the most reliable yet affordable custom DNA synthesis service among many competitors.

BioSecurity Statement:

Please note that ATG like other gene synthesis companies in compliance with bio-security relevant self-control have subjected themselves to a code of conduct, which is emerging to be an international quality management standard of the world wide community of Synthetic Biologists. Every Sequences is checked for its potential of being a biohazardous or having potential for its misuse in bioterrorism. Please if you know your gene has some potential, cooperate with the manufacturer or distributor e.g. ATG.

From Gene to Product - Use of Synthetic Genes, the Advantages

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Synthetic genes offer several advantages over cloned native DNA:

Change enzyme specificities and activities faster and easier by exactly specifying and designing the amino acid residues contributing to the catalytic center or ligand-binding domains. This will often enhance enzyme properties or performance.
Shuffle domains by combining functional protein domains of different origin and from different organisms. This can also be used to generate multi-purpose vaccines by combining epitopes from different sources in one target protein.
Overcome the limitatations of codon usage bias; for more details refer to the chapter on codon usage optimisation.
Attach or insert localisation signals to target your protein/nucleic acid to specific compartments or for secretion. This opens up avenues for engineering metabolic pathways in novel compartments.
Attach or modify protein-protein interaction domains in order to target your protein to specific reaction compartments.

Synthetic Genes and other double stranded DNA constructs produced by de novo DNA-synthesis

De novo gene synthesis offers a completely variable design of expressible and non-expressible DNA constructs. De novo gene synthesis enables you to tailor a natural or individually designed gene to your specific needs. You will be able to realize many different applications in Synthetic Biology within only a few weeks using Synthetic Genes. Think of all the possibilities you have:

Removal of secondary structures and repeat regions.
Adjustment of GC content to control the transcription rate or the function of a gene.
Recognition sites for DNA binding proteins (transcription factors, enhancer binding proteins, restriction enzymes) can be designed for use in gel mobility shift assays (EMSA) or DNAse I footprinting.
Mutated target genes - with random substitutions at one or more positions - can be of great impact in various diagnostic approaches.
Attachment of recognition or purification tags for in-vivo labelling and for facilitated affinity purification.
Insertion of protease cleavage sites.
Removal or addition of recognition sites for DNA restricton enzymes.
Natural DNA sequences that are only available in digital format.
Long hybridization probes that have been physically unavailable.

DNA - modifications

Applications:

Gene synthesis has found wide applications in molecular biology and biomedical research. From de novo gene synthesis, codon optimisation for protein overexpression, site-directed mutagenesis to DNA vaccine optimisation etc.

Often subcloning from cDNA is more difficult for rare genes with low copy number transcripts. Full length subcloning for larger genes is also problematic. At our ultra-low price, ATG:biosynthetics' Contract Gene Synthesis Service is the ideal substitution for your tedious subcloning efforts. With double strand sequencing and 100% accuracy guarantee you would speed up your research by focussing on more challenging aspects of your project.

Genes of high commercial values and academic interest from bacteria living in extreme environments or from virulent pathogens are hard to obtain due to the limited sources for bacterial cultures. Again our custom gene synthesis service puts your gene in your hand in just 3 weeks yet still within your tight budget.

Biochemists who need milligrams of proteins for their studies and cell biologists who depend on a readable signal of their gene in transfected cell lines perfectly understand the problems brought by low expression levels. Among many contributing factors, codon frequency and rare codon usage have been proven to be one of the parameters resulting in low expression level. With ATG:biosynthetics' customer DNA synthesis service you have the freedom of using only the most used codon for your particular expression host and have the potential of increasing your expression level. Furthermore, our gene synthesis service can provide you with a single gene that fits multiple host expressions. Be it E. coli and insect cell expression, yeast and human or E. coli and mouse, you decide what you want and we make it possible for you.

Are you a structural biologist trying hard to remove all of the cysteines from your favorite receptor or channel for a site-directed cysteine cross-link experiment? Still remove them one by one? We do it all at the same time even with codons optimised at your option.

ATG:Synthetic Genes and DNA Fragments - Improved Functionality for Your Synthetic Biology Applications

Please Order Your Synthetic Gene or DNA - Fragment here

ATG Guarantees 100% Sequence Identity and Highest Quality During all Processes of de novo Gene Synthesis and Gene Design in Order to Serve with the Highest Functionality Possible and Optimal Handling.

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