1. Sequence information (name and add a sequence)

Sequence name ? Please enter sequence name for sequence you copy-pasted. This name is not searched for in the databases (local database, NCBI Protein, NCBI Genebank). Please use the GI/accession field for data retrieval.

GI or accession (NCBI protein)  ? Please fill in either gi or accession (NCBI protein database) or paste sequence

or Protein sequence (Plain or FASTA format)

Length 

or DNA sequence (Plain or FASTA format)

Length  Start position Stop position 

Genetic code
1 Standard 2 Vertebrate Mitochondrial 3 Yeast Mitochondrial 4 Mold, Protozoan, and Coelenterate Mitochondria and the Mycoplasma Code 5 Invertebrate Mitochondrial 6 Ciliate, Dasycladacean and Hexamita Nuclear 9 Echinoderm Mitochondrial 10 Euplotid Nuclear 11 Bacterial Code 12 Alternative Yeast Nuclear 13 Ascidian Mitochondrial 14 Flatworm Mitochondrial 15 Blepharisma Nuclear 16 Chlorophycean Mitochondrial 21 Trematode Mitochondrial 22 Scenedesmus obliquus mitochondrial 23 Thraustochytrium Mitochondrial

2. Assembly information (create a library)

A-Centric

	Peptide length? Length of peptide (amino acids)	Peptide overlap? Overlap size e.g. 3 amino acids	Scanning span? Start and stop position of scan
			Start Stop
Create	or use preset definitions Epitope scanning library (length 12/overlap 4) Epitope scanning library (length 15/overlap 5) domain-size fragment interaction library		Clear

Centric

			Peptide length? Maximum (start length) and minimum (limit) peptide lengths in amino acids	Peptide overlap? N-Terminal and C-terminal overlaps which reduce the maximum peptide stepwise stopping above the limit (minimum length)	Scanning span? Start position of the maximum peptide, the stop position of the centric span is inserted automatically
	1		Max Min	N-Term. C-Term.	Start Stop
Create From selected overlapping centric regions only one will be shown					Clear