PepID and fusion tags:
PepID expression vectors can be equipped with various tags for protein purification, reporter analysis, specific localization, etc. The standard tag is GST (glutathion-S-transferase). Refer to the second box to see what tags can be offered.
The tags are designed into the expression vector, along with your resistance marker of choice (default: kanamycin)
Affinity and other tags:
| Tag | Length | Amino Acid Sequence | size (kDa) |
| Arg5-Tag | 5 (-6) | RRRRR | 0.8 |
| His6-Tag (Hexa-His) | 6 (2-10) | HHHHHH | 0.8 |
| Hat-Tag | 19 | KDHLIHNVHKEFHAHAHNK | 2.3 |
| V5-Peptide-Tag | 14 | GKPIPNPLLGLDST | 1.4 |
| Flag-Tag | 8-10 | DYKDDDDK | 1.0 |
| 3 x Flag-Tag | 22 | DYKDHDGDYKDHDIDYKDDDDK | 2.7 |
| Strep-Tag I | 9 | AWRHPQFGG | 1.0 |
| Strep-Tag II | 8 | WSHPQFEK | 1.1 |
| Nano-Tag9 | 9 | MDVEAWLGAR | 1.15 |
| Nano-Tag15 | 15 | MDVEAWLGARVPLVET | 1.8 |
| SBP-Tag | 38 | MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP | 4.3 |
| c-myc-Tag | 11 | EQKLISEEDL | 1.2 |
| S-Tag | 15 | KETAAAKFERQHMDS | 1.75 |
| Calmodulin-Binding-peptid | 26 | KRRWKKNFIAVSAANRFKKISSSGAL | 3.0 |
| Cellulose-Binding-Domain (variable) | 27-189 | MTVWGQCGGIGWSGPTNCAPGSACSTLNPYYAQCI | 3.0-20.0 |
| Chitin-Binding-Domaine | 51 |
TNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ
|
5.6 |
| GST-Tag | 211 |
LGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD
|
26.0 |
| Maltose Binding ProteinMalE-Gene, MBP-Tag | 396 |
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK
|
40.0 |
| Biotin Carboxyl Carrier Protein | 122 |
MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVMQQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAFIEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE
|
16.7 |
| Thioredoxin-Tag | 109 |
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
|
11.8 |
Applications:
More to come soon...
More to come soon...
Will be expanded...