ATG: synthetic genes, de novo DNA synthesis and more...Why spend time on difficult cloning work, tedious mutagenesis, sequencing, and troublesome gene design issues?
Synthetic genes and our top-notch synthetic bioinformatics expertise are the solution you are looking for!
Just electronically define your sequence of interest and let us do the rest. We will adapt codon usage for open reading frames and control regions, do the subcloning, work out finicky gene and gene cluster designs and deliver the final product to your doorstep.
- quality-checked, 100% sequence verified
- difficult and long templates, operons, multi-gene assemblies, etc.
- turnaround times as short as 7 to 10 business days; express service on demand
- 500+ customers reliably served in Europe and overseas, 13 years of experience
- top-notch service
- more than just genes: cloning, plasmid production, gene design & optimization, and more...
Your synthetic gene is more cost-efficient than you think! No costs for chemicals, cloning and restriction enzymes, manhours of work, instruction of interns, grad students or lab technicians. Just receive your gene construct and you are good to go.
Chemical synthesis of DNA: advantages, possibilities, promisesGene synthesis combines chemical synthesis of DNA molecules with traditional molecular biology approaches. A DNA template is produced in vitro by one of a multitude of techniques to concatenate oligonucleotides. The resulting gene construct is then cloned into a plasmid vector and amplified in microorganisms, usually specialized Escherichia coli laboratory strains.
Most notably, gene synthesis gives researchers the freedom of generating any gene in the absence of genomic or cDNA, simply based on sequences that have been defined in silico, e.g. through adapting existing "natural" sequences to a different codon usage or by building artificial genetic sequences from scratch by stitching together (fusing) elements from different DNA sources and/or organisms.
Gene synthesis is the method of choice for creating artificial gene sequences and eventually assembling artificial genomes out of it.
From Gene to Product - the Advantages of Using Synthetic Genes
Synthetic genes offer several advantages over cloned native DNA:
- Precisely define the sequence you need!
- Change enzyme specificities and activities faster and easier by exactly specifying and designing the amino acid residues contributing to the catalytic center or ligand-binding domains. This often enhances enzyme properties or performance.
- Shuffle domains by combining functional protein domains of different origin and from different organisms. This can also be used to generate multi-purpose vaccines by combining epitopes of different origin in one target protein.
- Overcome the limitatations of codon usage bias in heterologous protein expression.
- Attach or insert localization signals to target your protein/nucleic acid to specific compartments or the secretory pathway. This opens up avenues for engineering metabolic pathways in novel compartments.
- Attach or modify protein-protein interaction domains in order to target your protein to specific reaction compartments.
Synthetic Genes and other double stranded DNA constructs produced by de novo DNA-synthesis
De novo gene synthesis offers a completely variable design of expressible and non-expressible DNA constructs. De novo gene synthesis enables you to tailor a natural or individually designed gene to your specific needs. You will be able to realize many different applications in Synthetic Biology within only a few weeks using Synthetic Genes. Think of all the possibilities you have:
- Removal of secondary structures and repeat regions.
- Adjustment of GC content to control the transcription/translation rate (e.g. slow codon clusters for correct folding) or the function of a gene.
- Recognition sites for DNA binding proteins (transcription factors, enhancer binding proteins, restriction enzymes) can be designed for use in gel mobility shift assays (EMSA) or DNAse I footprinting.
- Mutated target genes - with random substitutions at one or more positions - can be of great impact in various diagnostic approaches.
- Attachment of recognition or purification tags for in-vivo labelling and for facilitated affinity purification.
- Insertion of protease cleavage sites.
- Removal or addition of recognition sites for DNA restricton enzymes.
- Natural DNA sequences that are only available in digital format.
- Long hybridization probes that have been physically unavailable.