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Expression |
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MultiGene |
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MultiLabel |
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FlexTec |
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Inquiry |
flexTEC systems - our basic Toggle systems:
The flexTEC systems are the basic implementation of the ToggleTec vector lay-out. These are ready-to-use with the appropriately designed gene cassettes.
ATG:biosynthetics GmbH has developed a vector-based gene assembly system with standardized modules that adhere to a uniform design logic. This system allows easy modification, exchange, addition, removal and regeneration of components or higher-order gene assemblies. This system has been de-signed with the idea of an industrial standard in mind and has successfully been implemented for multi-gene assemblies in metabolic engineering of bacteria.
tANCHOR:
tANCHOR technology: The new technology for exposing proteins on cell surfaces. The system is well suitable for peptide/ epitope/ protein display on the surface of mammalian cells applying two different members of the tetraspanine family of proteins, CD63 (HEK293T) and CD82 (HeLa) each expressed in various cell lines a little better. In addition the tANCHOR vectors are available with terminal mCHERRY fluorescence protein.Please feel free to download our tANCHOR Vector Kit .
flexColi Basic, flexColi Turbo
flexColi in its basic implementation is a system that lets you select from a variety of A-Box and S-Box functions (refer to table). The turbo versions are for high expression levels.The flexColi starter Kit includes T7 and lac - promoter variants, you may choose other advanced configurations as well. The flexColi transfer vectors consist of 2 acceptors and 4 donors:
| Acceptors | Donors | |
| flexColi A1-5 Acc: T7 prom, T7 terminator, Gent R | flexColi A1-5 Don1: T7 prom, T7 terminator, Kan/Neo R | |
| flexColi A1-6 Acc: lac prom, T7 terminator, Gent R | flexColi A1-6 Don1: lac prom, T7 terminator, Kan/Neo R | |
| flexColi A1-5 Don2: T7 prom, T7 terminator, Chl R | ||
| flexColi A1-6 Don2: lac prom, T7 terminator, Chl R |
Flip open more information (vector maps, etc.) by clicking on the Read more tag.
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| flexColi acceptor vectors (Click on vector map to expand!) | ||||||
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| flexColi donor vectors (Click on vector map to expand!) | ||||||
flexBACTurbo
flexBACTurbo in its basic implementation is a system that lets you select from two A-Box (with polh and p10 promoters, with/without Tn7 attachment sites) and three S-Box (KanR, GmR, CmR) functions you require (refer to table).In the basic (CLASSIC) set, TogBac transfer vectors come with 2 acceptors and 4 donors:
| Acceptors | Donors | |
| flexBAC A3.1.1: polh, SV40 terminator, Gent R | flexBAC A3.2.1KnR: polh, SV40 terminator, Kan R | |
| flexBAC A3.1.2: p10, HSV tk terminator, Gent R | flexBAC A3.2.2KnR: p10, HSV tk terminator, Kan R | |
| flexBAC A3.2.1CmR: polh, SV40 terminator, Chl R | ||
| flexBAC A3.2.2CmR: p10, HSV tk terminator, Chl R |
Flip open more information (vector maps, etc.) by clicking on the Read more tag.
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| flexBacTurbo acceptor vectors (Click on vector map to expand!) | ||||||
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| flexBacTurbo donor vectors (Click on vector map to expand!) | ||||||
Genes cloned into the transfer vectors can the be recombined into multi-gene cassette assemblies by Cre-loxP recombination or via toggle concatenation.
| Sample Cre-DeCre cascade of assembling and disassembling multi-gene (cassette) constructs. The red circles mark the loxP recombination signal(s). In this example, donors have an R6K gamma origin of replication but they may as well possess a ColE1 ori. |
All vectors host a split ori which allows conversion from a medium/high-copy ori (ColE1) into an R6K-gamma conditional ori by a simple restriction-ligation reaction.
Read more about the flexBACTurbo system in this downloadable PDF primer.