FlexColi advanced Kit – System Vectors:
For all E.coli Expression - A-BoX-Vectors the S-BoXes for: KmR, CmR and GmR are availlable| A0.0 (38 bp)- Vector AbsI(XhoI)-BssHII-SgrDI(SalI) | Basic Kit | 100 EUR |
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CE:P=Promotor=0
CE:O=Operator=0 CE:L=Leader=0 CDS:CDS=0, BssHII CE:T=Terminator=0 |
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| A1.1 (231 bp) AbsI(XhoI)-AscI-A1.1.-MauBI-SgrDI(SalI) | Advanced Kit | 150 EUR |
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ExpTogApp: ExpA1.1.
CE:P1=T7:promotor /BsrGI / CE:O2=LacO /SwaI / CE:L1=T7:Leader-Epsilon element /NdeI CDS: CA|TATG – CDS=0 – TGATC|A / BclI CE:T1=T7:terminator |
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| A1.2 (258 bp)AbsI(XhoI)-AscI-A1.2.-MauBI-SgrDI(SalI) | Advanced Kit | 150 EUR |
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CE=CIS:elements
ExpTogApp: ExpA1.2. CE:P1=S:Ptac–promotor /BsrGI / CE:O2=LacOid /SwaI / CE:L1=T7:Leader-Epsilon element /NdeI CDS: CA|TATG – CDS=0 – TGATC|A / BclI CE:T1=T7:Terminator |
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| A1.3 (253 bp)AbsI(XhoI)-AscI-A1.3.-MauBI-SgrDI(SalI) | Advanced Kit | 150 EUR |
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ExpTogApp: ExpA1.3.
CE:P1=S:Ptrc–promotor /BsrGI / CE:O2=LacO /SwaI / CE:L1=T7:Leader-Epsilon element /NdeI CDS: CA|TATG – CDS=0 – TGATC|A / BclI CE:T1=rrnB T1, rrnB T2:Terminator |
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| A1.4. (315 bp)AbsI(XhoI)-AscI-A1.4.-MauBI-SgrDI(SalI) | Advanced Kit | 150 EUR |
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ExpTogApp: ExpA1.4.
CE:P1=trc-lacUV5-promotor/BsrGI / CE:O2=LacO /SwaI / MCS: NdeI, NcoI, EcoRI, SacI, KpnI, Acc65I, BamHI, PstI CE:L1=T7:Leader-Epsilon element /NdeI CDS: CA|TATG – CDS=0 – TGATC|A / BclI CE:T1=rrnB T1, rrnB T2:Terminator |
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| A1.5. (278 bp)AbsI(XhoI)-AscI-A1.5.-MauBI-SgrDI(SalI) | Advanced Kit | 150 EUR |
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ExpTogApp: ExpA1.5.
CE:P1=T7-promotor/ CE:O2=LacO / CE:L1=T7:Leader-Epsilon element /NdeI CDS: CA|TATG – CDS=0 – TGATC|A / BclI CE:T1=T7:Terminator |
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| A1.6. (329 bp)AbsI(XhoI)-AscI-A1.6.-MauBI-SgrDI(SalI) | Advanced Kit | 150 EUR |
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ExpTogApp: ExpA1.6.
CE:P1=lac-promotor CE:O2=LacO CE:L1=Lac:Leader-Epsilon element /NdeI CDS: CA|TATG – CDS=0 – TGATC|A / BclI CE:T1=T7:Terminator |
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CE=Controll Element
P=Promoter
O=Operon
L=Leader
T=Terminator
MCS=Multiple Cloning Site
CDS=Coding Sequence
Vector Kits:
| A1.0-6. Kit ALL in ONE – Kit | Advanced Kit PARTIAL VECTORS | 499 EUR |
| all DONOR - vectors in one kit | |
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| A1.0-6. Kit ALL in ONE – Kit PLUS | Advanced Kit FULL VECTORS | 799 EUR |
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all DONOR - vectors in one kit - PLUS
all ACCEPTOR – vectors in one kit |
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| A1.0-6. Kit ALL in ONE – Kit PLUS PLUS | Advanced Kit ALL IN ONE KIT | 899 EUR |
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all DONOR - vectors in one kit PLUS bacterial stabs PLUS
all ACCEPTOR – vectors in one kit PLUS bacterial stabs |
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