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Expression |
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MultiGene |
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MultiLabel |
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Toggle |
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FlexTec |
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Inquiry |
Tools and Know-How for multi-protein and multi-peptide expression:
Protein but also peptide expression are centerpieces of experimental molecular biology as they allow generating bulk amounts of proteins that may otherwise occur only spuriously in the cell. These proteins may be generated in their native hosts but more commonly are over-expressed in heterologous hosts that are genetically more amenable than the organism they come from.
To achieve this, you will need:
- a plasmid-based system (or an alternative) to build your multi-gene expression constructs
- biocomputing expertise to allow adaptation of gene expression parameters to the host in question
- some design pointers
Multi-gene / protein expression systems
ATG's acceptor-donor plasmid systems for multigeneering (ToggleTec, MultiLabel) allow you to insert individual (1a) or multiple (1b) genes or gene cassettes into any single vector.
To generate multi-gene plasmid construct, you may either concatenate (2) them by toggling (described in more detail in the Toggle section) or other strategies, e.g. recombination-mediated processes (recombineering). The multi-gene constructs are then amplified in a bacterial host(3) and isolated (4) before transferring them to their target host (5). Ideally, the target host will then generate the desired proteins / protein complexes (right-hand end of image).
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Multi-peptide expression systems
The technology to build, host and express peptides and entire peptide libraries is described in the PepID section in considerable detail.
The technology to build, host and express peptides and entire peptide libraries is described in the PepID section in considerable detail.