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Dear Customers,
How to get your genes to work
in E. coli? Most easiest - convenient - fast and robust is to
use ECOS competent E.coli cells (DH5 α , JM109 )
- provided competent for the one minute minimum
handling protocol.
ECOS 1 minute competent cells are
availlable at: ATG: biosynthetics GmbH
please inquire. |
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ECOS Genotype
Table
|
Product |
Genotype |
Application |
|
ECOS Blue (XL1-Blue) |
rec A1 endA1
gyrA96 thi-1 hsdR17 supE44 relA1
lac (F'proAB lacIqZΔM15 Tn10 (Tetr)). |
Suitable for blue-white color
screening and routine cloning application. |
|
ECOS 21 / BL-21(DE3) |
F- ompT hsdSβ(rβ-mβ-) dcm gal
λ(DE3) |
Appropriate host for recombinant protein
expression using T7-based expression vectors. |
|
ECOS 101 (DH5α) |
endA1 recA1 relA1
gyrA96 hsdR17(rk-,
mk+) phoA
supE44 thi-1 Δ(lacZYA-argF)U169 Φ80
Δ(lacZ)M15 F- |
Suitable for cloning with large plasmid and
cDNA library construction, and also allow blue-white colonies
selection. |
|
ECOS 9-5
(JM109) |
e14-(McrA-) recA1 endA1
gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1
Δ(lac-proAB) (F traD36 proAB
lacIqZΔM15) |
Appropriate for blue-white color and robotic
screening. It is a fast growing strain, forming visible colonies
within 8 ~ 10 hours. |
ECOS Efficiency
Table
|
Product |
Strain |
Catlog
No# |
Stored
at -70℃
Efficiency |
Specification |
|
ECOS 101 Competent
Cells |
DH5α |
YE607 |
>1x108 |
100ulx10vials |
|
YE607-J |
100ulx80vials |
|
YE608 |
>3x108 |
100ulx10vials |
|
YE608-J |
100ulx80vials |
|
YE609 |
>1x109 |
100ulx10vials |
|
YE609-J |
100ulx80vials |
|
YE607-96 |
>1x108 |
40ulx96wells |
|
YE608-96 |
>3x108 |
40ulx96wells |
|
YE609-96 |
>1x109 |
40ulx96wells |
|
ECOS Blue Competent
Cells |
XL1-Blue |
YE107 |
>5x107 |
100ulx10vials |
|
YE107-J |
100ulx80vials |
|
YE108 |
>2x108 |
100ulx10vials |
|
YE108-J |
100ulx80vials |
|
YE109 |
>5x108 |
100ulx10vials |
|
YE109-J |
100ulx80vials |
|
YE107-96 |
>5x107 |
40ulx96wells |
|
YE108-96 |
>2x108 |
40ulx96wells |
|
YE109-96 |
>5x108 |
40ulx96wells |
|
ECOS 9-5 Competent Cells |
JM109 |
YE707 |
>5x107 |
100ulx10vials |
|
YE707-J |
100ulx80vials |
|
YE708 |
|
100ulx10vials |
|
YE708-J |
>1x108 |
100ulx80vials |
|
YE707-96 |
>5x107 |
40ulx96wells |
|
YE708-96 |
>1x108 |
40ulx96wells |
|
ECOS 21 Competent
Cells |
BL-21(DE3) |
YE207 |
>2x107 |
100ulx
5vials |
|
YE207-J |
100ulx40vials |
|
YE207-96 |
>2x107 |
40ulx96wells |
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