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flexTEC systems - our basic Toggle systems:


The flexTEC systems are the basic implementation of the ToggleTec vector lay-out. These are ready-to-use with the appropriately designed gene cassettes.

ATG:biosynthetics GmbH has developed a vector-based gene assembly system with standardized modules that adhere to a uniform design logic. This system allows easy modification, exchange, addition, removal and regeneration of components or higher-order gene assemblies. This system has been de-signed with the idea of an industrial standard in mind and has successfully been implemented for multi-gene assemblies in metabolic engineering of bacteria.

flexColi Basic, flexColi Turbo

flexColi in its basic implementation is a system that lets you select from a variety of A-Box and S-Box functions (refer to table). The turbo versions are for high expression levels.

The flexColi starter Kit includes T7 and lac - promoter variants, you may choose other advanced configurations as well. The flexColi transfer vectors consist of 2 acceptors and 4 donors:

Acceptors Donors
flexColi A1-5 Acc: T7 prom, T7 terminator, Gent R flexColi A1-5 Don1: T7 prom, T7 terminator, Kan/Neo R
flexColi A1-6 Acc: lac prom, T7 terminator, Gent R flexColi A1-6 Don1: lac prom, T7 terminator, Kan/Neo R
flexColi A1-5 Don2: T7 prom, T7 terminator, Chl R
flexColi A1-6 Don2: lac prom, T7 terminator, Chl R

Flip open more information (vector maps, etc.) by clicking on the Read more tag.
TogBac3.1.1 TogBac3.1.2
flexColi acceptor vectors (Click on vector map to expand!)
FC_A15_KnR FC_A16_KnR FC_A15_ChlR FC_A16_ChlR
flexColi donor vectors (Click on vector map to expand!)

flexBACTurbo

flexBACTurbo in its basic implementation is a system that lets you select from two A-Box (with polh and p10 promoters, with/without Tn7 attachment sites) and three S-Box (KanR, GmR, CmR) functions you require (refer to table).

In the basic (CLASSIC) set, TogBac transfer vectors come with 2 acceptors and 4 donors:

Acceptors Donors
flexBAC A3.1.1: polh, SV40 terminator, Gent R flexBAC A3.2.1KnR: polh, SV40 terminator, Kan R
flexBAC A3.1.2: p10, HSV tk terminator, Gent R flexBAC A3.2.2KnR: p10, HSV tk terminator, Kan R
flexBAC A3.2.1CmR: polh, SV40 terminator, Chl R
flexBAC A3.2.2CmR: p10, HSV tk terminator, Chl R

Flip open more information (vector maps, etc.) by clicking on the Read more tag.
TogBac3.1.1 TogBac3.1.2
flexBacTurbo acceptor vectors (Click on vector map to expand!)
TogBac3.2.1 TogBac3.2.2 TogBac3.2.1Chl TogBac3.2.2Chl
flexBacTurbo donor vectors (Click on vector map to expand!)

Genes cloned into the transfer vectors can the be recombined into multi-gene cassette assemblies by Cre-loxP recombination or via toggle concatenation.

Cre-Decre-cascade Sample Cre-DeCre cascade of assembling and disassembling multi-gene (cassette) constructs. The red circles mark the loxP recombination signal(s). In this example, donors have an R6K gamma origin of replication but they may as well possess a ColE1 ori.

All vectors host a split ori which allows conversion from a medium/high-copy ori (ColE1) into an R6K-gamma conditional ori by a simple restriction-ligation reaction.


Read more about the flexBACTurbo system in this downloadable PDF primer.