DNA and Peptide Libraries:Libraries of bio-molecules have become a useful tool in research and development, especially when intending to select a few relevant peptides, DNAs, or proteins from a gigantic pool of possible candidates. These libraries can either be completely random, i.e. the sequence is permutatated almost ad libitum, or rational, i.e. based on a given fixed sequence. Most focussed libraries will also be rational or semi-rational as the usually incorporate a great deal of information about the target structure, e.g. certain peptide motifs that are characteristic to B- or T-cell epitopes.
Construction, Hosting and Expression of Peptide Libraries in a Biological System:The development of analytical tools for detecting bacterial and viral agents as well as the matching vaccines requires a profound knowledge of the pathogen itself and of the immune processes in the host. Deciphering the genomes of numerous microorganisms and viruses as well as parallel intensive research into the mammalian immune system significantly contributed to understanding the complex, diverse immune processes in the healthy and sick organism.
These developments of the Omics era have opened up new avenues in vaccine research. Synthetic and systems biology have contributed novel methods and processes (gene synthesis, pathway engineering, metabolic modeling, etc.).
ATG:biosynthetics's bioPeptide technology PepID is such a development that will be presented here briefly.