PepID bio-based Peptide Libraries:
PepID combines strong computational algorithms for generating rationally designed, deterministic peptide libraries with a smart bio-based vector system for hosting and expressing DNA-encoded peptide libraries. The libraries are generally based on proteins that come from diagnostically and epidemiologically relevant bacteria and viruses, e.g flaviviruses. This makes them deterministic as the peptide sequences are based on existing and usually well-studied proteins.This sets it apart from chemically synthesized random libraries where peptide sequences are permutated without paying much attention to biological relevance.
PepID - how it works
PepID separates hosting and expression into 2 subsystems. It is a bio-peptide system because the peptides are produced in a biological system, usually a bacterial host organism (but it may be any other microorganism as well), from a plasmid DNA construct.Additional information and further reading:
For a quick run-through of our system, please take a look at this animated presentation.Click on the link and open/start the presentation. It will auto-play all the way through.
Please feel free to download our PepID quick sheet for an overview or a three-page article on the PepID technology.
An experimental application of PepID in research on diagnostic tools for West Nile virus infection has been published by Chabierski and colleagues in PLoS ONE.
DNA Optimization:
This is an aspect that should be considered as the peptides need to be expressible in E.coli along with eliminating any repeats, secondary structures, and the like that impede successful synthesis of the multi-peptide constructs. Read more in the section on Optimization.